Many clinical reports have demonstrated that the function-loss mutations in the SLC12A3 gene, mainly including Thr60Met, Asp486Asn, Gly741Arg, Leu859Pro, Arg861Cys, Arg913Gln, Arg928Cys, and Cys994Tyr, play the pathogenic effects in GS.[12] Our findings demonstrated that administration of using enema in GS patients may reduce the incidence of hypokalemia. This evidence concerns the gene SLC12A3 and Hypokalemia.