PLCG1 and viral infectious disease: A further study showed that neither Tubulin nor LaminA/C, a marker for the extractions of cytosol and nucleus, respectively, was readily detected by Western blot (Fig. 3C and D), which confirmed that the Golgi fractions from both MDBK and A549 cells were not contaminated by the cytosol protein and nuclear protein, which validated the findings that the virus infection increased the protein levels of p-PLC-γ1(S1248) in the Golgi apparatus.