Based on RT-qPCR analysis of tumor tissues and paracancerous normal tissues from 141 COAD patients, TRIM69 expression was significantly lower in tumor tissues than in non-malignant paracancerous normal tissues (Fig. 6A) and was markedly different in the cancer tissues of different pathological stages and different M stages (Fig. 6B-C), consistent with results found from analysis of COAD patient data from TCGA database (see Supplementary Figure S1 for detailed results for age, different T stages and different N stages). This evidence concerns the gene TRIM69 and colon adenocarcinoma.