INS and amenorrhea: These parameters encompassed basic biometric features (weight, BMI, WHR, reproductive function, F-G score, percentage with dominant follicles, regular menstruation, amenorrhea, oligomenorrhea, and polycystic ovaries according to Rotterdam criteria, sex-related hormones), biochemical profile of glucose metabolism (HbA1c, FBG, AUC-insulin, OGTT-insulin, and HOMA-IR), lipid profiles, liver/kidney function, and inflammation status.