Further pseudotime trajectory analysis confirmed this hypothesis (Fig. 5E, F): naïve IL7R+ CD4 cells have two differentiation routines, contributing to CXCL13+ CD4, CD40 LG+ CD4, and Treg cells (Fig. 5E–G), indicating the phenotypic transition of naïve CD4+ T cells to effector status may be an important phenomenon in CIP+ samples. Here, CXCL13 is linked to hereditary sensory and autonomic neuropathy.