Furthermore, the monocle (Fig. 3E) and slingshot (Fig. 3F) pseudotime trajectory analysis showed a possible differentiation trajectory from naïve IL7R+ CD8 into resident ZNF683+ CD8 and then into cytotoxic CD8+ T cell subsets: CXCL13+ CD8 and GZMB+ CD8 (Fig. 3F, G), which is consistent with the differentiation trajectory in which ZNF683+ CD8 contributes to cytotoxic CD8+ T cells in colitis induced by ICI therapy [30]. This evidence concerns the gene CD8A and colitis.