INS and hydrops fetalis: As a follow-up study, Chaurasia et al.100 performed mass spectrometry, quantifying 38 sphingolipids in BAT from mice fed a normal chow diet or HF diet, and found that Cer 16:0 levels are elevated upon HF diet feeding and that inhibition of Cer 16:0 synthesis in the liver led to improved glucose tolerance and insulin sensitivity along with reduced body fat in ob/ob mice148.