To address the role of FBXL6 in HCC metastasis in vivo, we generated Fbxl6LSL-fl/+ mice by inserting the CAG promoter-LoxP-STOP-LoxP-mouse Fbxl6 cDNA cassette into the ROSA26 locus in C57BL/6 N mice by CRISPR/Cas9-mediated genome editing. This evidence concerns the gene FBXL6 and hepatocellular carcinoma.