(4) Blocking PI3K-WNK1 signal pathway can reduce the hypersensitivity of mice with bone cancer pain to mechanical stimulation; (5) Blocking PI3K-WNK1 pathway can decrease the expression of IL-17 and TNF-α; (6) Blocking single PIC receptors (IL-17R and TNFR, etc.)in the brain may weaken the hypersensitivity of mice with bone cancer pain to mechanical stimulation by inhibiting PI3K-WNK1 signal. The gene discussed is TNF; the disease is bone neoplasm.