The PI3K/Akt pathway in the spinal dorsal horn of mice activates downstream WNK1, promotes the formation of bone cancer pain by up-regulating the level of inflammatory factors, inhibits Akt and blocks PI3K-WNK1 signal pathway, which can reduce the expression level of IL-17,TNF-α, increase the threshold of mechanical foot withdrawal and relieve bone cancer pain. This evidence concerns the gene TNF and bone neoplasm.