SYP and Alzheimer disease: In AD cases, triple colocalization of MAP2 with synaptophysin and astrocytes showed a 10-fold lower colocalization than synaptophysin with astrocytes (Figure 3D), and similarly MAP2 with synaptophysin and microglia showed a 44-fold lower colocalization compared with synaptophysin with microglia (Figure 3E), suggesting synaptic ingestion occurs predominantly in the absence of neurite ingestion.