To explore the role of BACH1 in angiogenesis, we first established 3D cultures of the human lung cancer cell lines A549 and H838; tumor organoids from mice with KRASG12D-induced lung cancer; and xenograft tumors from NSG mice injected s.c. with A549 cells (Figures 1A and Supplemental Figure 1, A–C; supplemental material available online with this article; https://doi.org/10.1172/JCI169671DS1). This evidence concerns the gene BACH1 and lung cancer.