TGFB1 and neoplasm: Quantitative PCR (qPCR) of whole tumor lysate for transcript levels of the cytokines TGFβ (Tgfb1–2), IL10 (Il10), and IL35 (Ebi3 and Il12a), associated with suppressed antitumor immunity (25), showed reduced levels in Vegfr2Y1173F/+ tumors (Figure 2N).