To mimic E11 skipping in human cancer tissues and understand the biological functions of p73γ, CRISPR-Cas9 technology was used to generate stable H1299 and Mia-PaCa2 cell lines in that the splicing acceptor for TP73 exon 11 (E11) was deleted by using two guide RNAs (Figure 2—figure supplement 1A). The gene discussed is TP73; the disease is cancer.