To establish if CD8+ T lymphocytes were systemically recognizing their tumor-associated cognate antigen, IFNγ-enzyme-linked immunosorbent spot (ELISpot) assays were performed using splenocytes from treated mice, as indicated in Figure 5A. Images of IFNγ-ELISpot wells (Figure 5C) and quantitative data (Figure 5D) show that the numbers of CD8+ T cells recognizing the canonical ovalbumin (OVA) epitope (SIINFEKL) presented by H-2Kb were clearly increased. This evidence concerns the gene IFNG and neoplasm.