The positive ratio of senescent HSCs over α-SMA-positive areas continued to increase from 2 to 9 months, reaching a peak of 40.41% at 9 months and decreased at 12 months (22.86%) when precancerous lesions occurred and further decreased at 14 months (14.58%) when finally progressed to HCC (Figure 1F). Here, ACTA1 is linked to hepatocellular carcinoma.