Clusters of activated HSCs were observed at both 5 and 9 months of HFCD-HF/G feeding, however there exists difference that senescent cells started to appear at 2 months (Figure 1E) and became much more at 5 months in areas surrounded with activated HSCs (Figure 1E); whilst number of senescent myofibroblast-like cells reached the highest in these α-SMA-positive areas at 9 months (Figure 1E). The gene discussed is ACTA1; the disease is hydrops fetalis.