Almost all reported or clinically used probes in intraoperative guidance such as Aminopeptidase N-activatable YH-APN (30 min) and “always-on” ICG (14 days) require more time to achieve adequate TNR 47, decreasing their practicality for routine or real-time clinical use, while NBD mediated image occurred within 3 min of topically spraying the tumor not only in animals but also in clinical tissues (video S1 and S2). This evidence concerns the gene ANPEP and neoplasm.