We successfully designed primers and probes for six CRC‐specific markers (FAM72A, FAM72B, LIFR, OSMR, ZNF264 and ZNF543) covering 10 of 15 CpGs for further testing the methylation level of cfDNA with ddPCR‐based assays (Figure 3A; Supporting information). The gene discussed is FAM72A; the disease is colorectal carcinoma.