TCIRG1 and nonpapillary renal cell carcinoma: To determine whether TCIRG1 affected the growth of ccRCC cell lines, we used small interfering RNA (siRNA) to knockdown TCIRG1 in OS-RC-2 and 769-P cells, screened out si-TCIRG1#1 and si-TCIRG1#2 through RT-qPCR (Supplementary Figure S2K), and verified the knockdown efficiency using Western blot (Fig. 5C, D).