Concurrently, the expression of gluconeogenesis‐, fatty acid biosynthesis‐ and fatty acid oxidation‐associated factors were examined with RT‐qPCR and western blot and it was noticed that HMGCR, FABP1, FAS expression was augmented while CPT1A expression was cut down in the liver tissues of NAFLD mice upon exposure to HFD treatment, which was all restored when Asprosin was downregulated (Figure 4D,E). This evidence concerns the gene FAS and metabolic dysfunction-associated steatotic liver disease.