These patient-derived cells have beenshown to contain a high proportion of tumor-initiating cells (TICs),to stain positive for SOX2 and nestin, and show the ability to self-renewas well as differentiate into various neural cell fates.31 FACS experiments demonstrated that p-HTMI reproduciblydetected between 70 and 90% of the cells in these three cultures within10 min from application of the molecule (Figure 3b, c, and e). This evidence concerns the gene NES and neoplasm.