We found a significant enrichment of mis-spliced events in ASD-risk genes from the SFARI (OR = 1.6, FDR = 6.6 × 10−11), MSSNG-2017 (OR = 3.3, FDR = 1.3 × 10−6) and MSSNG-2022 (OR = 2.6, FDR = 3.6 × 10−7) studies in the DM1 brain organoid, including previously identified DMD miE (Fig. 3g–h). Here, DMD is linked to myotonic dystrophy type 1.