We next investigated whether the mutTDP43 mature astrocytes recapitulate a critical pathological hallmark of ALS/FTD by displaying an abnormal TDP43 localization, including nuclear depletion and cytoplasmic accumulation/deposition in neurons and glia cells (Neumann et al., 2006; Geser et al., 2008; Zhang et al., 2013; Gregory et al., 2020; Baughn et al., 2023). Here, TARDBP is linked to amyotrophic lateral sclerosis.