In addition, in HEK293T cells with both ACE2 and AXL knockout, overexpression of AXL alone can significantly promote viral infection, while the addition of soluble recombinant AXL protein or recombinant NTD protein can inhibit AXL-mediated viral infection, which further confirms that AXL-mediated SARS-CoV-2 infection cannot depend on ACE2. The gene discussed is AXL; the disease is viral infectious disease.