Besides, some pathways differed between subclusters; for instance, CD8 + cluster 7 and CD4 + cluster 8 showed strong IFN-γ and IFN-α responses, enhanced complement activities, high allograft rejection activities (Fig. 2d) and granzyme expressions of GZMA, GZMM and GZMK (Fig. 2e), indicating these two subclusters of T cells showed some certain anti-tumor activities. The gene discussed is GZMA; the disease is neoplasm.