To investigate the altered immunophenotype of neutrophils, flow cytometry was subsequently applied to detect the expression of the degranulation markers CD11b (ITGAM) and CD66b (CEACAM8) on the surface of neutrophils, as well as ICAM-1 (CD54) and CXCR4 (CD184), which were commonly used as indicators of tumor-associated neutrophils phenotypes. Here, CXCR4 is linked to neoplasm.