To examine whether NDUFS1 affects the metastasis ability of GC cells, we analyzed the effect of NDUFS1 overexpression or knockdown on GC cell migration and invasion in vitro and lung metastasis in vivo in athymic BALB/c nude mice using NDUFS1-overexpressing MKN45/AGS and NDUFS1-interfering N87/HGC-27 GC cells. This evidence concerns the gene NDUFS1 and gastric cancer.