In summary, our study provided the first evidence (Fig. 6g) that the loss of NDUFS1 expression in GC cells was conducive to the activation of mROS-HIF1α signaling pathway and the induction of transcriptional expression of HIF1α downstream genes such as GLUT1, VEGFA, and FBLN5, which are responsible for the malignant progression of GC. The gene discussed is NDUFS1; the disease is gastric cancer.