WB (Fig. 5a) and IHC (Fig. 5b) analyses showed that overexpression of NDUFS1 inhibited the expression of FBLN5 in MKN45 GC cells in vitro and in vivo in athymic nude mice (P < 0.05), whereas knockdown of NDUFS1 enhances the expression of FBLN5 in N87 GC cells and xenografts (P < 0.05). Here, FBLN5 is linked to gastric cancer.