All six-month-old (n = 9) Pten null prostates develop high-grade prostate intraepithelial neoplasia (HGPIN) phenotype- the main precursor lesion to adenocarcinoma53, and they displayed a marked increase in p-MED1 staining, along with higher MYC, p-AKT and p-S6 staining - a characteristic pattern associated with LCMT1 loss/reduction (Supplementary Fig. 7f). This evidence concerns the gene MYC and prostate intraepithelial neoplasia.