The results showed that deletion of NRBF2 decreased sucrose preference in SPT and increased the immobility time in both TST and FST (Fig. 3b–d), with no difference of locomotor activity and center exploration in open field test (OFT) (Fig. 3e; Supplemental Fig. S6a–c), suggesting that deletion of NRBF2 gene leads to the depression-like phenotype, but not anxiety-like behavior, of mice. This evidence concerns the gene NRBF2 and depressive disorder.