NAT10 and major depressive disorder: Pharmacological methods prevented NAT10’s anti-anxiety and antidepressant-like effects.369 As one of the most abundant modifications on tRNA and rRNA, m5C is enzymatically catalyzed by members of the NOL1/NOP2/SUN domain (NSUN) family and DNA methyltransferase homolog DNMT2.370 By altering the translational dynamics of Glycine (Gly)-rich synaptic proteins, the impaired cortical NSUN2, which is a tRNA methyltransferase, exerts bidirectional impacts on tRNA methylation and induces depression-like behavior in the mouse brain.371