In terms of different grades of glioma, cytoplasmic staining of TRPM7 was significantly increased in grade IV GBM compared with that in grade II glioma (p < 0.05) (Fig. 7B left), while nuclear staining of TRPM7 was significantly increased in grade IV GBM compared wth that of grade II gliomas (p < 0.05) (Fig. 7B right). The gene discussed is TRPM7; the disease is central nervous system cancer.