Integrated metabolomics and proteomics analysis was performed, and six key proteins, DENN domain containing 3, Ras protein‐specific guanine nucleotide releasing Factor 2 (RasGRF2), potassium voltage‐gated channel subfamily Q member 2, sprouty RTK signaling antagonist 2, unc‐5 netrin receptor C and glutathione S‐transferase alpha 1, were identified in the MGMT group and GBM group. The gene discussed is RASGRF2; the disease is glioblastoma.