ALS is associated with cytosolic aggregates containing specific misfolded proteins in both neuronal and glial cells that may lead to disease initiation and propagation, including SOD1 aggregates in SOD1 familial ALS and TDP43 aggregates in C9orf72 and most other forms of familial ALS, as well as SALS.170. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.