To further investigate the effect of AQP3 on gallbladder injury in vivo, we constructed a mouse model of cholelithiasis overexpressing AQP3. The mRNA expression of AQP3 was notably down-regulated in the gallbladder tissues of mice with cholelithiasis, which could be increased after transduction with a lentiviral vector harboring oe-AQP3 (Fig. 7A). This evidence concerns the gene AQP3 and cholelithiasis.