Meanwhile, ELISA results showed that TNF-α, IL-1β and IL-18 expression was significantly up-regulated in gallbladder tissues of the mice with cholelithiasis relative to that in the normal control mice; overexpression of AQP3 inhibited the expression of TNF-α, IL-1β, and IL-18 in gallbladder tissues of the mice with cholelithiasis (Fig. 7C). The gene discussed is TNF; the disease is cholelithiasis.