In this regard, the aim of this in vivo experiment was to determine the therapeutic effect of SLN containing streptomycin (STR) and hydroxychloroquine (HCQ) (STR-HCQ-SLN) on acute and chronic phases of brucellosis and its effect on serum levels of C-reactive protein, vitamin D, renal function parameters and liver enzymes. This evidence concerns the gene CRP and brucellosis.