SMPD3 and acute myeloid leukemia: Costaining of AML-12 cells confirmed the observations from HepG2 cells, namely that in the PAL-treated cells, nSMase2 localized significantly to the PM (Fig. 3G for single cells and supplemental Fig. S3 for lower magnification), based on the clear overlap between the CD44 and nSMase2 signal.