MYC and osteosarcoma: To address this, we utilized three separate cancer cell lines we previously characterized to have intact molecular clocks that are disrupted by overexpressed MYC or N-MYC: SHEP N-MYC-ER (neuroblastoma), SKNAS N-MYC-ER (neuroblastoma), and U2OS MYC-ER (osteosarcoma) [31,32].