As expected, sPD-1 binding was detected in SKNAS cells treated with PBS and the control isotype while in tumor cells that have been previously treated with Atezolizumab loss of streptavidin signal was detected, demonstrating that masking of PD-L1 prevented sPD-1 binding to its molecular target (Figure 1D, right panel). This evidence concerns the gene HOXD13 and neoplasm.