Previous research studies have reported thatcertain subpopulations in colon cancer express distinct surface biomarkers,including CD133, CD44, and CD26, which are indicative of stem cell-likeproperties.5,40,41 To isolate colon CSCs in our study, we used CD133 and CD44 surfaceantigens, which have previously been characterized as reliable markersfor identifying CSCs.31,42. This evidence concerns the gene PROM1 and colonic neoplasm.