In this study, we (1) identified the proportion of a rural population with different grades of cervical intraepithelial neoplasia (CIN), (2) isolated and identified cervicovaginal pathogens within the microenvironment of the participants, and (3) estimated and compared the concentrations of IL-4, IL-6, IL-10, TNF-α, and INF-γ among participants with CIN to know the trend of the immune response. The gene discussed is TNF; the disease is cervical squamous intraepithelial neoplasia.