This study therefore (1) identified the proportion of the rural population with different grades of cervical intraepithelial lesions (CIN), (2) isolated and identified cervicovaginal pathogens within the microenvironment of the participants, and (3) estimated and compared the concentrations of IL-4, IL-6, IL-10, TNF-α, and INF-γ of participants with CIN to the normal population. The gene discussed is TNF; the disease is cervical squamous intraepithelial neoplasia.