In tumor sample DD0194a from another dog, we can predict that there has been biallelic somatic inactivation of MSH2, through frameshift mutation and loss of heterozygosity, as the allele frequency (AF) of the frameshift was 0.87 and this and 4 other upstream mutations have an AF of between 0.89 and 0.96 and fall near or within a predicted > 1 Mb deletion. Here, MSH2 is linked to neoplasm.