The cytotoxicity of CD133 CAR-T and PD-1 s cells to the CD133-high-expression HCC cell line Hep3B was significantly enhanced compared with that of CD133 specific CAR-T and Mock T cells at an E:T of ratio 0.25:1 overnight (P = 0.002 and P < 0.0001; Fig. 3E, F). The gene discussed is PROM1; the disease is hepatocellular carcinoma.