PROM1 and neoplasm: The CD3-stained area in CD133 CAR-T and PD-1 s cell-treated mice (4/5) was higher than that in CD133-specific CAR-T-treated (0/5) and Mock T-cell-treated mice (0/5) (Fig. 5F, G).To evaluate the antitumour efficiency of CD133 and PD1s CAR-T cells in a metastasis mouse model, a metastasis xenograft mouse model was established by injecting 2.5 million transfected Hep3B-luc cells into NCG mice until BLI showed a stable tumour followed by administering T cell therapy 55 days later (Fig. 6A).