The results showed that only the conditional medium from LoVo cells, a cell line derived from metastatic nodules resected from a male patient with grade IV Dukes C CRC, specifically induced TCAF2 upregulation in TPCNM (Figure S9, Supporting Information), indicating the TCAF2 expression in TPCs was not correlated with KRAS‐ or BRAF‐mutations in CRC cells, which might be associated with the high‐metastatic property of tumor cells. Here, TCAF2 is linked to colorectal carcinoma.