TCAF2 and neoplasm: To further determine the role of TCAF2+ TPCs in tumor metastasis in vivo, HCT116 or DLD‐1 cells pre‐mixed with TPCNMVector, TPCNMTCAF2, TPCLMshNC or TPCLMshTCAF2 were orthotopically transplanted into the cecum wall of nude mice to construct liver metastatic xenografts (Figure 3E).