Despite similar tumor mutational burden, metastatic sites displayed significantly higher ITH than primary ASCs (P = 0.0059 by Wilcoxon rank-sum test) and the similar result was observed in the EGFR-positive ASCs subgroup (P = 0.018) (Fig. 3b and Supplementary Fig. 3a, b). The gene discussed is EGFR; the disease is neoplasm.