Firstly, the in vivo immunogenic cell death activation was investigated by immunofluorescence staining of CRT expression in the primary tumor sections, and a strong red fluorescence was observed in the irradiated ADPAH@AOT group, which was 5.5- and 6.2-fold relative to those of ADPAH@AOT and PBS group, respectively (Fig. 5e and Supplementary Fig. 22). The gene discussed is CALR; the disease is neoplasm.