To determine whether STUB1 is involved in the effect of CRTAC1 on the chemosensitivity of NSCLC, three shRNA plasmids targeting different STUB1 sites were stably transfected into H1299 cells with overexpressed CRTAC1 to knock down STUB1 and then knockdown of STUB1 restored Akt1 protein expression (Fig. 5F). This evidence concerns the gene STUB1 and non-small cell lung carcinoma.