To further characterize the biological functions of SPDEF in luminal BC cells, we firstly found that luminal BC cells, including MCF7, T47D and BT-474, had elevated SPDEF expression in comparison with MCF10A cells using RT-qPCR (Fig. 2A and Figure S1A) and western blot (Fig. 2B, C and Figure S1B, C). The gene discussed is SPDEF; the disease is breast cancer.