To explore the role of miR-297-mediated PTBP3 in HCC cell functions in relation to PI3K/AKT signaling pathway, the expression of PTBP3, p-PI3K, AKT and p-AKT were evaluated by western blot in HepG2 and Huh7 cells after the transfection of miR-297 mimics, miR-297 inhibitor. The gene discussed is PTBP3; the disease is hepatocellular carcinoma.