Furthermore, in order to quantify the proliferation level of rat prostates, we detected the nuclear antigen Ki-67, which is positively correlated with cell proliferation activity, and immunofluorescence staining of the rat prostate tissues showed that the positive rate of Ki-67 was significantly increased in BPH rats (p < 0.01) and was inhibited by RSL3 or TRO treatment (p < 0.05). The gene discussed is MKI67; the disease is benign prostatic hyperplasia.