Furthermore, T treatment significantly increased the levels of iron (Fig. 11F) and MDA (Fig. 11G), while decreased the levels of SOD2, CAT (Fig. 11E and Additional file 11: Fig. S7C) and GSH (Fig. 11H) in the rat prostate, indicating that the level of ferroptosis in the prostate of T-induced BPH rats was elevated, which could be further aggravated by RSL3 (Fig. 11E–H). Here, SOD2 is linked to benign prostatic hyperplasia.