Meanwhile, IHC staining and Western blot experiments suggested that T treatment could up-regulate the levels of LC3B (Fig. 11I, K, M and Additional file 11: Fig. S7D) and Beclin1 (Fig. 11J, L, M, Additional file 11: Fig. S7D) in T-BPH rat prostates, which could be exacerbated by RSL3 treatment and partially reversed by TRO. The gene discussed is BECN1; the disease is benign prostatic hyperplasia.