Also, IHC staining and Western blot results showed that the expression of GPX4 (Fig. 11A, C, E and Additional file 11: Fig. S7C) and Nrf2 (Fig. 11B, D, E and Additional file 11: Fig. S7C) decreased in T-BPH rat prostate and further decreased after RSL3 treatment, while could be rescued by TRO treatment. This evidence concerns the gene TRO and benign prostatic hyperplasia.