Moreover IHC staining showed that the expression of GPX3 decreased in T-BPH rats (p < 0.05), and TRO treatment significantly upregulated the expression of GPX3 (p < 0.05) but there was no significant change with RSL3 treatment (p > 0.05) (Fig. 10G, H). The gene discussed is GPX3; the disease is benign prostatic hyperplasia.