Transfection of these mini-replicons or infection with ZIKV mini-rep SRIPs revealed the crucial involvement of ZIKV MTase domain of NS5 and NS4B in facilitating efficient RdRp-mediated self-replication of the ZIKV mini-replicon-driven RNAs, leading to a significant expression of the heterogeneous genes. The gene discussed is RAF1; the disease is infection.