To demonstrate the potential modulatory effects of rchIL-17A in MD, real-time PCR analysis was used to assess the differential expression of genes (COX-2, IL-10, IL-17A, TGF-β, IL-12p40, and IFN-γ) in spleens at 21-dpi (Figure 7). Here, TGFB1 is linked to Menkes disease.