MCF7, HEPG2, and NCIH460 cancer cells, as well as kidney cells (HEK293) expressing EGFR and MET receptors, were subjected to varying concentrations (62.5, 125, 250, and 500 μg/mL) of SPIONs, BS-S, BS-SPP, and Butein to predict its cytotoxic effect by MTT dye reduction assay [36]. This evidence concerns the gene EGFR and cancer.